Joshua’s IBF, WBA and WBO belts will all be on the line on Saturday night at Tottenham Hotspur Stadium when he takes on the former undisputed cruiserweight champion of the world, one of his toughest tests in the boxing ring to date.
Usyk, who took to the stage wearing a shirt that read “I’m coming for you’, weighed in at 221lbs - a career heaviest by four pounds.
Speaking after the weigh-in, two-time heavyweight champion AJ told Sky Sports, "It's just a fight, we just do our best. I respect the man, he's achieved phenomenal things.
"I don't really watch my weight. I don't know why there is this big thing about weight?
"There is no game-plan, just win, because you never know what is going to happen!"
Elsewhere, WBO cruiserweight world champion Lawrence Okolie weighed in at 199lbs ahead of the first defence of his crown, while his opponent Dilan Prasovic came in at a pound heavier at 200lbs.
“I don't really think about belts. I just want to get a massive knockout," ‘The Sauce’ told Sky Sports.
Callum Smith, fighting for the first time since his defeat to Canelo in December and under the tutelage of new trainer Buddy McGirt, came in at the light-heavyweight limit of 175lbs, as did his weekend opponent Lenin Castillo.
"It's my time to show those improvements. Saturday night I will show where I want to be," Smith said.
Florian Marku had to be escorted off stage after shoving when he came face-to-face with Maxim Prodan at Thursday’s press conference. The weigh-in was a little more subdued, although still clearly tense, as Prodan weighed in at 146lbs, while Marku weighed in four pounds over the limit and has two hours to lose the weight.
Lightweight Campbell Hatton, son of British boxing legend Ricky, is hoping to make it four wins from four since turning pro in March when he takes on Sonny Martinez. Both men weighed in at 136lbs.
Anthony Joshua is 2/5 to beat Oleksandr Usyk with Betfred, while the Ukrainian is 9/4 to spring an upset. The draw is 20/1.