Barca have not lost a home game in the Champions League for five years yet the Spurs boss says his players should head to Catalonia full of confidence.
"Barcelona are one of the best teams in Europe and they have unbelievable players," said Pochettino. "We have big respect for Barcelona, but we have belief that we can win."
Last night, Spurs kept their Champions League qualification hopes alive with a hard-earned win over Inter Milan at Wembley. A second-half goal from Christian Eriksen proved enough for Spurs in a 1-0 win, a game they knew they had to win to stay alive in the competition.
"I think the performance was very good, we fully deserved the victory," said Pochettino. "We scored at the end but did everything to try to beat a very good team. Inter Milan showed a great level, a team that's so difficult to beat. The players deserve the credit, the performance was very good. We dominated, created the best chances and of course now we're in a position that depends on us.
"If we can win at Barcelona, we're going to go through to the next stage of the Champions League. But of course it will be so difficult and we need to focus now on Sunday – another derby against Arsenal. We cannot stop. We need to stay humble, try to improve because football is about always improving. But we're happy. Chelsea and now Inter and both games were must win games. And now we're going to play another game that we must win."
When pushed on his bold comments about winning in Barcelona, he said: "All is possible in football. We only need to show again a good level to compete at our best. To arrive at Barcelona and compete in our best we need to have fresh legs, fresh minds, no injuries. To arrive in that way in Barcelona we need to manage and we need to rotate the squad. If not the demands of the Premier League are so tough.
"We're playing Champions League and the cup against Arsenal at the Emirates. Every three days playing games is so tough - so tough for us but for our players so so difficult. They're not going to have one day off until January."